Laboratory Biosaftety Cabinet Class II Type A2 And Class II Type B2

Class II Type A2/B2 Biological Safety Cabinet

A biological safety cabinet (BSC) is a box-shaped, negative pressure air purification safety equipment that can stop some potentially harmful biological particles from evaporating during experimental operations. In the domains of microbiology, biomedicine, genetic engineering, and the manufacturing of biological products, it is extensively employed in scientific study, instruction, clinical inspection, and production. It is the most fundamental piece of safety protection gear in the laboratory biosafety first-level protective barrier.

Biological Safety Cabinet Operation:

The high-efficiency particulate air filter (HEPA) in the outside air filters the outside air, which is how the biological safety cabinet operates. It maintains negative pressure within the cabinet and uses vertical airflow to safeguard the workers. Additionally, the cabinet’s air must be filtered by HEPA filter and then discharged into the atmosphere to protect the environment.

Principles for selecting biological safety cabinets in biosafety laboratories:

It is often not essential to employ a biological safety cabinet or a class I biological safety cabinet when the laboratory level is 1. When working with infectious materials, a Class II biological safety cabinet with partial or full ventilation should be utilized; when the laboratory level is Level 2, a Class I biological safety cabinet can be used when microbial aerosols or splashing activities may occur; Only Class II-B complete exhaust (Type B2) biological safety cabinets should be used while working with chemical carcinogens, radioactive materials, and volatile solvents. A fully exhausted Class II-B (Type B2) or Class III biological safety cabinet should be utilized for any procedures involving infectious materials when the laboratory level is Level 3. A level III complete exhaust biological safety cabinet should be utilized when the laboratory level is level 4. When employees are wearing positive pressure protection equipment, Class II-B biological safety cabinets can be employed.

Biosafety Cabinets (BSC), also known as Biological Safety Cabinets, offer personnel, product, and environmental protection through laminar airflow and HEPA filtration for the biomedical/microbiological lab.

Biological safety cabinets generally consist of two parts: a box body and a bracket. The box body mainly includes the following structures:

1. Air Filtration System

The most crucial mechanism for ensuring this equipment’s functionality is the air filtration system. It is made up of an exterior exhaust air filter, a driving fan, an air duct, and four air filters in total. Its major purpose is to continually bring in clean air, ensuring that the work area’s downdraft (vertical airflow) flow rate is not less than 0.3 m/s and that the cleanliness level is guaranteed to be 100 grades. In order to avoid environmental contamination, the external exhaust flow is also cleansed concurrently.

The HEPA filter is the main working portion of the system. Its frame is made of a unique fireproof material, and corrugated aluminum sheets split it into grids. These grids are filled with emulsified glass fiber sub-particles, and the filter’s efficiency can reach 99.99% to 100%. Pre-filtering and purifying the air before it enters the HEPA filter is made possible by the pre-filter cover or pre-filter at the air input, which can increase the HEPA filter’s lifespan.

2. External exhaust air box system

The outer exhaust box system is made up of an exhaust duct, a fan, and an outside exhaust box shell. To safeguard the samples and experimental objects in the cabinet, the external exhaust fan extracts the dirty air from the workspace with the help of an external exhaust filter. To safeguard the operator, the air in the work area is allowed to leave.

3. Sliding front window drive system

The sliding front window drive system is composed of front glass door, door motor, traction mechanism, transmission shaft and limit switch.

4. The lighting source and UV light source are located on the inside of the glass door to ensure a certain brightness in the working room and to sterilize the table and air in the working room.

5. The control panel has devices such as power supply, ultraviolet lamp, lighting lamp, fan switch, and controlling the movement of the front glass door. The main function is to set and display the system status.

Class II A2 biological safety cabinet/biological safety cabinet manufactory’s main characters: 1. Air curtain isolation design prevents internal and external cross-contamination, 30% of the air flow is discharged outside and 70% of the internal circulation, negative pressure vertical laminar flow, no need to install pipes.

2. The glass door may be opened and closed entirely for sterilization, and the placement height restriction alert signals. It can also be adjusted up and down and placed wherever.3. For the operator’s convenience, the power output socket in the work area is outfitted with a waterproof socket and a sewage interface.4. To reduce emission pollution, a specific filter is fitted at the exhaust air.5. The workspace is constructed of premium 304 stainless steel that is seamless, sleek, and devoid of dead ends. It may stop the erosive compounds and disinfectants from eroding and is simple to disinfect fully.6. It adopts LED LCD panel control and built-in UV lamp protection device, which can only be opened when the safety door is closed.7. With DOP detection port, built-in differential pressure gauge.8, 10° tilt angle, in line with the human body design concept